{"id":1036,"date":"2021-01-26T10:38:34","date_gmt":"2021-01-26T10:38:34","guid":{"rendered":"https:\/\/info.ias.tuwien.ac.at\/bsc\/?p=1036"},"modified":"2021-01-26T10:44:46","modified_gmt":"2021-01-26T10:44:46","slug":"cell%e2%80%90free-in-vitro-reduction-of-carboxylates-to-aldehydes-with-crude-enzyme-preparations-to-a-key-pharmaceutical-building-block","status":"publish","type":"post","link":"https:\/\/info.ias.tuwien.ac.at\/bsc\/publications\/cell%e2%80%90free-in-vitro-reduction-of-carboxylates-to-aldehydes-with-crude-enzyme-preparations-to-a-key-pharmaceutical-building-block\/","title":{"rendered":"Cell\u2010free in vitro reduction of carboxylates to aldehydes: With crude enzyme preparations to a key pharmaceutical building block"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"1036\" class=\"elementor elementor-1036\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-40b6b534 elementor-section-full_width elementor-section-height-default elementor-section-height-default\" data-id=\"40b6b534\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-default\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-144c6f67\" data-id=\"144c6f67\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-3270c25b elementor-widget elementor-widget-text-editor\" data-id=\"3270c25b\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t<p><img fetchpriority=\"high\" decoding=\"async\" class=\"aligncenter size-large wp-image-1045\" src=\"https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-content\/uploads\/2021\/01\/26012021-post-scheme-1024x544.png\" alt=\"\" width=\"1024\" height=\"544\" srcset=\"https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-content\/uploads\/2021\/01\/26012021-post-scheme-1024x544.png 1024w, https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-content\/uploads\/2021\/01\/26012021-post-scheme-300x159.png 300w, https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-content\/uploads\/2021\/01\/26012021-post-scheme-768x408.png 768w, https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-content\/uploads\/2021\/01\/26012021-post-scheme-36x19.png 36w, https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-content\/uploads\/2021\/01\/26012021-post-scheme-48x25.png 48w, https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-content\/uploads\/2021\/01\/26012021-post-scheme-60x32.png 60w, https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-content\/uploads\/2021\/01\/26012021-post-scheme.png 1356w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/p><p>The scarcity of practical methods for aldehyde synthesis in chemistry necessitates the development of mild, selective procedures. Carboxylic acid reductases catalyze aldehyde formation from stable carboxylic acid precursors in an aqueous solution. Carboxylic acid reductases were employed to catalyze aldehyde formation in a cell\u2010free system with activation energy and reducing equivalents provided through auxiliary proteins for ATP and NADPH recycling. In situ product removal was used to suppress over\u2010reduction due to background enzyme activities, and an N\u2010protected 4\u2010formyl\u2010piperidine pharma synthon was prepared in 61% isolated yield. This is the first report of preparative aldehyde synthesis with carboxylic acid reductases employing crude, commercially available enzyme preparations.<\/p><p><img decoding=\"async\" class=\"aligncenter size-large wp-image-1044\" src=\"https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-content\/uploads\/2021\/01\/26012021-post-scheme-1-1024x527.png\" alt=\"\" width=\"1024\" height=\"527\" srcset=\"https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-content\/uploads\/2021\/01\/26012021-post-scheme-1-1024x527.png 1024w, https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-content\/uploads\/2021\/01\/26012021-post-scheme-1-300x154.png 300w, https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-content\/uploads\/2021\/01\/26012021-post-scheme-1-768x395.png 768w, https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-content\/uploads\/2021\/01\/26012021-post-scheme-1-36x19.png 36w, https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-content\/uploads\/2021\/01\/26012021-post-scheme-1-48x25.png 48w, https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-content\/uploads\/2021\/01\/26012021-post-scheme-1-60x31.png 60w, https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-content\/uploads\/2021\/01\/26012021-post-scheme-1.png 1282w\" sizes=\"(max-width: 1024px) 100vw, 1024px\" \/><\/p><p>DIO: <a href=\"https:\/\/doi.org\/10.1002\/biot.202000315\" target=\"_blank\" rel=\"noopener\">https:\/\/doi.org\/10.1002\/biot.202000315<\/a><\/p><p><strong>Keywords:<\/strong><br \/>aldehyde, biocatalysis, carboxylic acid reductase (CAR), in vitro cofactor recycling, pharma synthon<br \/><strong>Authors:<\/strong><br \/>Anna Schwarz, Sebastian Hecko, Florian Rudroff, Jeffrey T. Kohrt, Roger M. Howard, Margit Winkler<\/p>\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>The scarcity of practical methods for aldehyde synthesis in chemistry necessitates the development of mild, selective procedures. Carboxylic acid reductases catalyze aldehyde formation from stable carboxylic acid precursors in an aqueous solution. Carboxylic acid reductases were employed to catalyze aldehyde formation in a cell\u2010free system with activation energy and reducing equivalents provided through auxiliary proteins [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[5],"tags":[8],"_links":{"self":[{"href":"https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-json\/wp\/v2\/posts\/1036"}],"collection":[{"href":"https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-json\/wp\/v2\/comments?post=1036"}],"version-history":[{"count":0,"href":"https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-json\/wp\/v2\/posts\/1036\/revisions"}],"wp:attachment":[{"href":"https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-json\/wp\/v2\/media?parent=1036"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-json\/wp\/v2\/categories?post=1036"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-json\/wp\/v2\/tags?post=1036"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}