{"id":2096,"date":"2021-08-16T21:13:43","date_gmt":"2021-08-16T21:13:43","guid":{"rendered":"https:\/\/info.ias.tuwien.ac.at\/bsc\/?p=2096"},"modified":"2021-08-16T21:16:44","modified_gmt":"2021-08-16T21:16:44","slug":"chemo-enzymatic-cascade-for-the-generation-of-fragrance-aldehydes","status":"publish","type":"post","link":"https:\/\/info.ias.tuwien.ac.at\/bsc\/publications\/chemo-enzymatic-cascade-for-the-generation-of-fragrance-aldehydes\/","title":{"rendered":"Chemo-Enzymatic Cascade for the Generation of Fragrance Aldehydes"},"content":{"rendered":"\t\t<div data-elementor-type=\"wp-post\" data-elementor-id=\"2096\" class=\"elementor elementor-2096\" data-elementor-post-type=\"post\">\n\t\t\t\t\t\t<section class=\"elementor-section elementor-top-section elementor-element elementor-element-40c6719 elementor-section-full_width elementor-section-height-default elementor-section-height-default\" data-id=\"40c6719\" data-element_type=\"section\">\n\t\t\t\t\t\t<div class=\"elementor-container elementor-column-gap-no\">\n\t\t\t\t\t<div class=\"elementor-column elementor-col-100 elementor-top-column elementor-element elementor-element-7cb721af\" data-id=\"7cb721af\" data-element_type=\"column\">\n\t\t\t<div class=\"elementor-widget-wrap elementor-element-populated\">\n\t\t\t\t\t\t<div class=\"elementor-element elementor-element-29443d08 elementor-widget elementor-widget-text-editor\" data-id=\"29443d08\" data-element_type=\"widget\" data-widget_type=\"text-editor.default\">\n\t\t\t\t<div class=\"elementor-widget-container\">\n\t\t\t\t\t\t\t\t\tPublication Title: Chemo-Enzymatic Cascade for the Generation of Fragrance Aldehydes\r\n\r\nby Daniel Schwendenwein, Anna K. Ressmann, Marcello Entner, Viktor Savic, Margit Winkler,* and Florian Rudroff*\r\n\r\n<em>Catalysts<\/em>\u00a0<b>2021<\/b>,\u00a0<em>11<\/em>(8), 932;\u00a0<a href=\"https:\/\/doi.org\/10.3390\/catal11080932\">https:\/\/doi.org\/10.3390\/catal11080932<\/a>\r\n<div class=\"html-article-content\">\r\n<div id=\"abstract\" class=\"abstract_div\">\r\n<div class=\"html-abstract-title\">\r\n<h2>Abstract<\/h2>\r\n<\/div>\r\n<div class=\"art-abstract in-tab hypothesis_container\">In this study, we present the synthesis of chiral fragrance aldehydes, which was tackled by a combination of chemo-catalysis and a multi-enzymatic in vivo cascade reaction and the development of a highly versatile high-throughput assay for the enzymatic reduction of carboxylic acids. We investigated a biocompatible metal-catalyzed synthesis for the preparation of \u03b1 or \u03b2 substituted cinnamic acid derivatives which were fed directly into the biocatalytic system. Subsequently, the target molecules were synthesized by an enzymatic cascade consisting of a carboxylate reduction, followed by the selective C-C double bond reduction catalyzed by appropriate enoate reductases. We investigated a biocompatible oxidative Heck protocol and combined it with cells expressing a carboxylic acid reductase from\u00a0<span class=\"html-italic\">Neurospora crassa<\/span>\u00a0(<span class=\"html-italic\">Nc<\/span>CAR) and an ene reductase from\u00a0<span class=\"html-italic\">Saccharomyces pastorianus<\/span>\u00a0for the production fragrance aldehydes.<\/div>\r\n<div class=\"art-keywords in-tab hypothesis_container\"><em>Keywords:\u00a0<\/em><a href=\"https:\/\/www.mdpi.com\/search?q=chemo-enzymatic%20cascade\">chemo-enzymatic cascade<\/a>;\u00a0<a href=\"https:\/\/www.mdpi.com\/search?q=carboxylic%20acid%20reductase\">carboxylic acid reductase<\/a>;\u00a0<a href=\"https:\/\/www.mdpi.com\/search?q=ene%20reductase\">ene reductase<\/a>;\u00a0<a href=\"https:\/\/www.mdpi.com\/search?q=Heck-coupling\">Heck-coupling<\/a>;\u00a0<a href=\"https:\/\/www.mdpi.com\/search?q=fragrance%20aldehydes\">fragrance aldehydes<\/a><\/div>\r\n<\/div>\r\n<\/div>\r\n<div class=\"hypothesis_container\">\r\n<div class=\"html-body\"><\/div>\r\n<\/div>\t\t\t\t\t\t\t\t<\/div>\n\t\t\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/div>\n\t\t\t\t\t<\/div>\n\t\t<\/section>\n\t\t\t\t<\/div>\n\t\t","protected":false},"excerpt":{"rendered":"<p>Publication Title: Chemo-Enzymatic Cascade for the Generation of Fragrance Aldehydes by Daniel Schwendenwein, Anna K. Ressmann, Marcello Entner, Viktor Savic, Margit Winkler,* and Florian Rudroff* Catalysts\u00a02021,\u00a011(8), 932;\u00a0https:\/\/doi.org\/10.3390\/catal11080932 Abstract In this study, we present the synthesis of chiral fragrance aldehydes, which was tackled by a combination of chemo-catalysis and a multi-enzymatic in vivo cascade reaction and [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"site-sidebar-layout":"default","site-content-layout":"default","ast-site-content-layout":"","site-content-style":"default","site-sidebar-style":"default","ast-global-header-display":"","ast-banner-title-visibility":"","ast-main-header-display":"","ast-hfb-above-header-display":"","ast-hfb-below-header-display":"","ast-hfb-mobile-header-display":"","site-post-title":"","ast-breadcrumbs-content":"","ast-featured-img":"","footer-sml-layout":"","theme-transparent-header-meta":"default","adv-header-id-meta":"","stick-header-meta":"default","header-above-stick-meta":"","header-main-stick-meta":"","header-below-stick-meta":"","astra-migrate-meta-layouts":"default","ast-page-background-enabled":"default","ast-page-background-meta":{"desktop":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"ast-content-background-meta":{"desktop":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"tablet":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""},"mobile":{"background-color":"var(--ast-global-color-5)","background-image":"","background-repeat":"repeat","background-position":"center center","background-size":"auto","background-attachment":"scroll","background-type":"","background-media":"","overlay-type":"","overlay-color":"","overlay-opacity":"","overlay-gradient":""}},"footnotes":""},"categories":[5],"tags":[8],"_links":{"self":[{"href":"https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-json\/wp\/v2\/posts\/2096"}],"collection":[{"href":"https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-json\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-json\/wp\/v2\/comments?post=2096"}],"version-history":[{"count":0,"href":"https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-json\/wp\/v2\/posts\/2096\/revisions"}],"wp:attachment":[{"href":"https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-json\/wp\/v2\/media?parent=2096"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-json\/wp\/v2\/categories?post=2096"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/info.ias.tuwien.ac.at\/bsc\/wp-json\/wp\/v2\/tags?post=2096"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}