Rudroff Lab

Chemo-Enzymatic Cascade for the Generation of Fragrance Aldehydes

Publication Title: Chemo-Enzymatic Cascade for the Generation of Fragrance Aldehydes by Daniel Schwendenwein, Anna K. Ressmann, Marcello Entner, Viktor Savic, Margit Winkler,* and Florian Rudroff* Catalysts 2021, 11(8), 932; https://doi.org/10.3390/catal11080932 Abstract In this study, we present the synthesis of chiral fragrance aldehydes, which was tackled by a combination of chemo-catalysis and a multi-enzymatic in vivo cascade reaction and …

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Enzyme Cascade Design and Modelling

In our book “Enzyme Cascade Design and Modelling” we have joined our forces and brought a team of experts in multi-enzymatic and chemo-enzymatic cascades! Editors: Kara, Selin, Rudroff, Florian (Eds.) Book Highlights Creates the basis for pathway design via the concept of ‘biocatalytic retrosynthesis’ Enhances the readers’ understanding on kinetic modelling and reaction engineering Introduces process design, …

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Cell‐free in vitro reduction of carboxylates to aldehydes: With crude enzyme preparations to a key pharmaceutical building block

The scarcity of practical methods for aldehyde synthesis in chemistry necessitates the development of mild, selective procedures. Carboxylic acid reductases catalyze aldehyde formation from stable carboxylic acid precursors in an aqueous solution. Carboxylic acid reductases were employed to catalyze aldehyde formation in a cell‐free system with activation energy and reducing equivalents provided through auxiliary proteins …

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Biocatalysis in Green and Blue: Cyanobacteria

Recently, several studies have proven the potential of cyanobacteria as whole-cell biocatalysts for biotransformation. Compared to heterotrophic hosts, cyanobacteria show unique advantages thanks to their photoautotrophic metabolism. Their ability to use light as energy and CO2 as carbon source promises a truly sustainable production platform. Their photoautotrophic metabolism offers an encouraging source of reducing power, …

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Pyrazines: Synthesis and Industrial Application of these Valuable Flavor and Fragrance Compounds

Alkyl pyrazines—other than being extracted from various natural sources such as coffee beans, cocoa beans, and vegetables—can be synthesized by chemical methods or by certain microorganisms. The importance of pyrazines for the food industry is expected to grow in the upcoming years due to the higher demand for convenience products. The roasty, nutty, and earthy …

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Boosting photobioredox catalysis by morpholine electron donors under aerobic conditions

Light-driven reduction of flavins, e.g. FAD or FMN, by sacrificial electron donors emerged as a convenient method to promote biocatalytic transformations. However, flavin activation has been restricted to oxygen-free conditions to prevent enzyme deactivation caused by reactive oxygen species (ROS). Herein, we show that the photoreduction of FMN by morpholines, including 3-(N-morpholino)propanesulfonic acid (MOPS), lessens the deactivation …

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BioZone Project by Florian Rudroff approved by FWF

We are happy to announce that Florian Rudroff was granted FWF Project with title, Biologisch inspirierte Alkenspaltung – BioZone The key scientific challenges are the following: Find a biochemical substitute to the well-established chemical ozonolysis. Development of chemical probes to screen Nature’s huge sequence space efficiently and accurately. Novel biocatalyst: broad substrate scope, use a …

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